Journal: Scientific reports

Article Title: Chimeric yellow fever 17D-Zika virus (ChimeriVax-Zika) as a live-attenuated Zika virus vaccine.

doi: 10.1038/s41598-018-31375-9

Figure Lengend Snippet: Figure 1. CYZ-yfs and CYZ-hs (lead) candidates. (A) Predicted signalase cleavage scores by SignalP3.0 software for parental viruses, YF 17D and wt ZIKV, and the CYZ chimeras. ZIKV-specific residues are in bold. Several additional CYZ variants were constructed containing additional attenuating genetic modifications, such as CYZ-dC (3-amino acid deletion in C) and CYZ-dg (de-glycosylated E protein) variants based on CYZ-hs (see text for details). (B) Plaque morphologies of CY-yfs, CYZ-hs, and YF 17D parent virus in Vero cells. Plaques were stained with Crystal violet on day 5 post-infection. (C) Growth curves of CYZ-yfs and CYZ-hs viruses in serum-free Vero cell culture at indicated MOIs.

Article Snippet: 9SCIentIFIC RepoRts | (2018) 8:13206 | DOI:10.1038/s41598-018-31375-9 Titers of viruses were determined in Vero cells either using a standard plaque assay on Day 5 post-infection with crystal violet staining24,32 or by immunostaining of foci as previously described49 with anti-ZIKV E protein MAb 0502156 (Aalto Bioreagents, Dublin, Ireland) and anti-mouse IgG Fc-HRP conjugated secondary antibody (31437; Thermo Fisher Scientific, Waltham, MA).

Techniques: Software, Construct, Virus, Staining, Infection, Cell Culture